In Villa Lina, an eclectic mansion from the early 1900s, six themed rooms display a selection from one of the most important private collections of Hispano-Guaraní sacred art: saints, Virgins and Christ figures carved in wood and painted by Guaraní and European artists in the missions and colonial towns. The blend of Indigenous and European features in many of the figures is striking. The museum also has an auditorium, a café, a shop and a terrace that hosts concerts and plays.

It was created by the foundation of collector Nicolás Darío Latourrette Bo, who gathered more than 700 pieces of 17th- and 18th-century Hispano-Guaraní Baroque sacred art. It was inaugurated on March 24, 2010, and opened to the public three days later in the Villa Lina mansion.